We create an unique approach that is non-destructive into the embryo for molecular intercourse recognition of embryonic specimens. Embryonic bloodstream through the inside of this eggshell was swabbed onto a FTA ® Elute Micro Card (Whatman) just after egg dissection. DNA had been extracted after the manufacturer’s guidelines with a protocol adjusted for automatic analysis that is high-throughput the Eppendorf EPmotion 5075 liqu >® card extractions of adult P. vitticeps bloodstream examples (letter = 30).
We then carried out a PCR-based test, which will be diagnostic when it comes to existence regarding the W chromosome. PCR conditions used Holleley et al. 14; however, as a result of the probability of low DNA levels from embryonic product, we increased the amount of DNA included with PCRs (3 µl per response; around 65 ng DNA per PCR). 继续阅读“We developed Molecular intercourse recognition in embryonic samples”